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              技術文章

              MCM2 Antibody Catalog Number:OM284350

              點擊次數:32 發布時間:2024/9/27 14:31:02
               

              Product Profile

              Product Name MCM2 Antibody
              Antibody Type Primary Antibodies
              Product description
              The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.1) Yu, Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206.
              More 
              Immunogen
              Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM2.

              Key Feature

              Clonality Polyclonal
              Host Species Rabbit
              Tested Applications

              ELISAWB

               
              MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification Protein A

              Target Information

              Gene Symbol MCM2
              Alternative Names
              MCM2
              BM28
              CCNL1
              CDCL1
              cdc19
              D3S3194
              MITOTIN
              More 
              Molecular Weight(MW) 99 kDa

              Database Links

              Entrez Gene 4171
              Protein Accession NP_004517

              Application

              • Application

                Antibody used in WB on mouse neural stem at 1:1000.

              • Application

                Antibody used in WB on Human Jurkat 1.25 ug/ml.

              Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
              Application Notes
              MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

              Additional Information

              Form Liquid
              Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
              Storage Buffer IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

              原創作者:上海篤瑪生物科技有限公司

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