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              技術文章

              MCM2 Antibody Catalog Number:OM284353

              點擊次數:40 發布時間:2024/9/26 14:48:49
               

              Product Profile

              Product Name MCM2 Antibody
              Antibody Type Primary Antibodies
              Product description
              MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.1) Saade, E., et al. Proteomics 9(21):4934-4943(2009)
              More 
              Immunogen
              This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
              Species Reactivity

              HumanMouse

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol MCM2
              Alternative Names
              DNA replication licensing factor MCM2
              Minichromosome maintenance protein 2 homolog
              Nuclear protein BM28
              MCM2
              BM28
              CCNL1
              CDCL1
              KIAA0030
              More 
              Molecular Weight(MW) 102 kDa
              Sequence Similarities Predicted species reactivity based on immunogen sequence: Xenopus

              Database Links

              Entrez Gene 4171
              Protein Accession P49736

              Application

              • Application

                MCM2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

              • Application

                Western blot analysis of MCM2 Antibody in HL-60 cell line lysates (35ug/lane)

              • Application

                Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.

              Application Notes
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
               

              原創作者:上海篤瑪生物科技有限公司

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