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聯系人：楊燕燕
技術文章
Prohibitin antibody Catalog Number：OM270566
Product Profile
| Product Name | Prohibitin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Cat, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (94%), Xenopus laevis (89%), Dog (100%), Cat (100%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (5) |
| Cellular Localization | Mitochondrion inner membrane |
Application
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Application
Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
-
Application
Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000
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Application
Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000
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Application
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.
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Application
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.
-
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Prohibitin antibody at 1:500 dilution.
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Application
Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE diluted at 1:1000
| Positive Control | IMR32 , U87-MG , Neuro2A , GL261 , C8D30 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
原創作者：上海篤瑪生物科技有限公司
