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              技術文章

              Anti-PHB1 antibody Catalog Number:OM642067

              點擊次數:39 發布時間:2024/9/26 14:35:12
               

              Product Profile

              Product Name Anti-PHB1 antibody
              Antibody Type Primary Antibodies
              Immunogen
              Recombinent Proteins

              Key Feature

              Clonality Monoclonal
              Isotype IgG
              Host Species Mouse
              Tested Applications

              ELISAICC/IFIHCWB

               
              WB:1:200-1:2000
              IHC:1:200-1:1000
              ICC/IF:1:100-1:500
              Species Reactivity

              HumanRabbit

              Concentration 1 mg/ml
              Purification Protein A

              Target Information

              Gene Symbol PHB
              Gene Synonyms
              PHB1
              HEL-215
              HEL-S-54e
              Gene Full Name prohibitin
              Gene Summary
              This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
              More 
              Molecular Weight(MW) 30 kDa
              Source Mouse
              Cellular Localization Cell membrane, Cylasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

              Application

              • WB

                Western blot analysis using PHB1 mouse mAb against HT1080 (1), MCF-7 (2), NIH 3T3 (3), K562 (4) and COS-7 (5) cell lysate.

              • IHC

                Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PHB1 antibody with DAB staining.

              • ICC/IF

                Immunofluorescence analysis of PC-3 cells using PHB1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

              Application Notes
              WB:1:200-1:2000
              IHC:1:200-1:1000
              ICC/IF:1:100-1:500

              Additional Information

              Form Liquid
              Storage Instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
              Storage Buffer Purified antibody in PBS with 0.05% sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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