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聯系人：楊燕燕
技術文章
Prohibitin Antibody Catalog Number：OM288898
點擊次數：61 發布時間：2024/9/26 14:19:12
Product Profile
| Product Name | Prohibitin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1). |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | PHB |
|---|---|
| Alternative Names | |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Database Links
| Entrez Gene | 5245 |
|---|---|
| Protein Accession | P35232 |
Application
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Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).
-
Application
Formalin-fixed ａnd paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
-
Application
Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell ( histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
原創作者：上海篤瑪生物科技有限公司
