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              技術文章

              G6PD antibody Catalog Number:OM253796

              點擊次數:42 發布時間:2024/9/25 15:21:21
               

              Product Profile

              Product Name G6PD antibody
              Antibody Type Primary Antibodies
              Product description
              Rabbit Polyclonal antibody to G6PD (glucose-6-phosphate dehydrogenase)This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
              More 
              Immunogen
              Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary.

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              *Optimal dilutions/concentrations should be determined by the researcher. :
              Species Reactivity

              HumanMouseRat

              Predicted Reactivity Xenopus laevis, Rabbit, Sheep, Rhesus Monkey, BovineXenopus laevis (82%), Rabbit (95%), Sheep (92%), Rhesus Monkey (99%), Bovine (95%)
              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Synonyms
              G6PD1 antibody
              G6PD antibody
              "glucose-6-phosphate dehydrogenase
              G6PD antibody"
              glucose-6-phosphate 1-dehydrogenase antibody
              glucose-6-phosphate dehydrogenase antibody
              More 
              Molecular Weight(MW) 59 kDa (note) (2)

              Application

              • Application

                Immunohistochemical analysis of paraffin-embedded U87 xenograft, using G6PD antibody at 1:300 dilution.

              • Application

                Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with G6PD antibody diluted at 1:500.

              • Application

                Sample (30 ug of whole cell lysate) A: HeLa S3 7.5% SDS PAGE diluted at 1:500

              Positive Control 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
              Application Notes
              *Optimal dilutions/concentrations should be determined by the researcher. :

              Additional Information

              Form Liquid
              Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

              原創作者:上海篤瑪生物科技有限公司

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