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              • 企業類型:生產商
              • 注冊資金:人民幣萬

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              聯系人:楊燕燕

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              技術文章

              G6PD Catalog Number:OM638966

              點擊次數:51 發布時間:2024/9/25 15:20:03
               

              Product Profile

              Product Name G6PD
              Antibody Type Primary Antibodies
              Product description
              Glucose-6-phosphate 1-dehydrogenase (G6PD) plays an important role in the pentose phosphate pathway. It is a member of the glucose-6-phosphate dehydrogenase family of proteins. G6PD is a ubiquitous enzyme that produces pentose sugars for nucleic acid synthesis, but is also involved in carbohydrate degradation, as it is one of the main producers of NADPH reducing power. G6PD has NADP as a co-factor and structural element. It can be found as a homodimer or homotetramer, and is primarily detected in lymphoblasts, granulocytes and sperm. Defects in G6PD can cause chronic non-spherocytic hemolytic anemia (CNSHA), especially in areas in which malaria is an epidemic. Individuals with a high level of G6PD-deficiency are at higher risk of acute hemolytic attacks.
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              Immunogen
              Recombinant protein.

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              WBICCIHC

               
              WB:1:500~1:2000
              ICC:1:200~1:500
              IHC:1:50~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 
              Species Reactivity

              HumanMouseRat

              Concentration 1 mg/mL.

              Target Information

              Alternative Names
              G6PD antibody G6PD_HUMAN antibody G6PD1 antibody G6pdx antibody Glucose 6 phosphate 1 dehydrogenase antibody Glucose 6 phosphate dehydrogenase antibody Glucose 6 phosphate dehydrogenase
              G6PD antibody Glucose-6-phosphate 1-dehydrogenase antibody MET19 antibody POS10 antibody Zwf1p antibody
              More 
              Molecular Weight(MW) 59 kDa
              Cellular Localization Cylasm. Nucleus. Membrane.

              Database Links

              SwissProt ID P11413
              Q00612
              P05370

              Application

              • Application

                Fig1: Western blot analysis of G6PD on different lysates using anti-G6PD antibody at 1/1,000 dilution. Positive control:Lane1: A549Lane2: HelaLane3: MCF-7Lane4: PC-12Lane 5: Mouse spleen

              • Application

                Fig2: ICC staining G6PD in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

              • Application

                Fig3: ICC staining G6PD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

              • Application

                Fig4: ICC staining G6PD in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

              • Application

                Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-G6PD antibody. Counter stained with hematoxylin.

              • Application

                Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-G6PD antibody. Counter stained with hematoxylin.

              • Application

                Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-G6PD antibody. Counter stained with hematoxylin.

              • Application

                Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-G6PD antibody. Counter stained with hematoxylin.

              Positive Control A549, Hela, MCF-7, PC-12, mouse spleen tissue lysate, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse testis tissue.
              Application Notes
              WB:1:500~1:2000
              ICC:1:200~1:500
              IHC:1:50~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 

              Additional Information

              Form Liquid
              Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
              Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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