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              技術文章

              G6PD Antibody Catalog Number:OM279344

              點擊次數:43 發布時間:2024/9/25 15:16:44
               

              Product Profile

              Product Name G6PD Antibody
              Antibody Type Primary Antibodies
              Product description
              G6PD encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia.1) Louicharoen, C., et al. Science 326(5959):1546-1549(2009)
              More 
              Immunogen
              This G6PD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human G6PD.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              FACSIHC-PWB

               
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
              Species Reactivity

              Human

              Concentration 1 mg/ml

              Target Information

              Gene Symbol G6PD
              Alternative Names
              Glucose-6-phosphate 1-dehydrogenase
              G6PD
              G6PD
              Molecular Weight(MW) 59 kDa

              Database Links

              Entrez Gene 2539
              Protein Accession P11413

              Application

              • Application

                Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

              • Application

                Western blot analysis of G6PD Antibody in K562,MCF-7 cell line lysates (35ug/lane)

              • Application

                G6PD Antibody IHC analysis in formalin fixed and paraffin embedded testis followed by peroxidase conjugation of the secondary antibody and DAB staining.

              Application Notes
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
               

              原創作者:上海篤瑪生物科技有限公司

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