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技術文章
Rabbit anti-MBP polyclonal antibody - middle region Catalog Number：OM116494
Product Profile
| Product Name | Rabbit anti-MBP polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-MBP antibody: synthetic peptide directed towards the middle region of human MBP |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 More IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | BovineDogHorseHumanMousePigRat |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | MBP |
|---|---|
| Gene Synonyms | |
| Gene Full Name | Myelin basic protein |
| Gene Summary | The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation ａnd stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, ａnd may also be involved in signaling pathways in T-cells ａnd neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. MBP induces T-cell proliferation.The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes ａnd Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow ａnd the immune system. These mRNAs arise from the long MBP gene (otherwise called ＇Golli-MBP＇) that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli ａnd the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts ａnd gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons ａnd produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit ａnd that this arrangement is important for the function and/or regulation of these genes. More |
| Alternative Names | |
| Molecular Weight(MW) | 33kDa |
| Sequence | 304 amino acids |
Database Links
| Entrez Gene | 4155 |
|---|---|
| SwissProt ID | P02686 |
| Protein Accession | NP_001020272 |
Application
-
Immunofluorescence
500 ug rat brain homogenate
Amount of IP Antibody :
6 ug
Primary Antibody :
MBP
Primary Antibody Dilution :
1:500
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:5000
Gene Name :
MBP
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science -
Western blot
IP Suggested Anti-MBP Antibody Positive Control: NT2 CELL/BRAIN TISSUE
-
Immunohistochemical
Human brain stem cells
Primary Antibody Dilution :
1:500
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:1000
Color/Signal Descriptions :
MBP: Red DAPI:Blue
Gene Name :
MBP
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science -
Western blot
0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human brain
| Application Notes | WB:1:500~1:2000 More IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot ａnd store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
原創作者：上海篤瑪生物科技有限公司
