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              技術文章

              NQO1 antibody , C-term Catalog Number:OM265893

              點擊次數:60 發布時間:2024/9/24 15:06:26
               

              Product Profile

              Product Name NQO1 antibody , C-term
              Antibody Type Primary Antibodies
              Product description
              Rabbit Polyclonal antibody to NQO1 (NAD(P)H dehydrogenase, qui 1)This gene is a member of the NAD(P)H dehydrogenase (qui) family and encodes a cylasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quis to hydroquis. This protein's enzymatic activity prevents the one electron reduction of quis that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
              More 
              Immunogen
              Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NQO1. The exact sequence is proprietary.

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              ICC/IFIHC-PWB

               
              WB:1:500~1:2000
              ICC/IF:1:100~1:500
              IHC-P:1:50~1:200
              Species Reactivity

              HumanMonkeyMouseRat

              Predicted Reactivity Rhesus MonkeyRhesus Monkey (100%)
              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification Affinity purified

              Target Information

              Gene Synonyms
              DHQU antibody
              DIA4 antibody
              DTD antibody
              NMOR1 antibody
              NMORI antibody
              QR1 antibody
              NQO1 antibody
              phylloqui reductase antibody
              dioxin-inducible 1 antibody
              azoreductase antibody
              NAD(P)H:Qui acceptor oxidoreductase type 1 antibody
              qui
              More 
              Molecular Weight(MW) 31 kDa (note) (4)
              Cellular Localization Cylasm

              Application

              • Application

                Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

              • Application

                NQO1 antibody [C2C3], C-term detects NQO1 protein at cylasm in human rectal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human rectal carcinoma. NQO1 antibody [C2C3], C-term diluted at 1:500.

              • Application

                NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis. A. 50 µg mouse heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

              • Application

                NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis. A. 50 µg rat heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

              • Application

                Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NQO1 antibody at 1:200 dilution.

              Positive Control Molt-4 , Raji , mouse heart , rat heart
              Application Notes
              WB:1:500~1:2000
              ICC/IF:1:100~1:500
              IHC-P:1:50~1:200

              Additional Information

              Form Liquid
              Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

              原創作者:上海篤瑪生物科技有限公司

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