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技術文章
NQO1 [13A1] Catalog Number：OM628315
Product Profile
| Product Name | NQO1 [13A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within Human NQO1 aa 1-285. |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:1,000-1:5,000: IHC:1:50-1:100: | |
| Species Reactivity | HumanRat |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Cylasm. |
Database Links
| SwissProt ID | P15559 P05982 |
|---|
Application
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Application
Fig1: Western blot analysis of NQO1 on different lysates. Proteins were transferred to a PVDF membrane ａnd blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated
-
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the antibody (EM1801-21) at 1/50 dilution, for 30 minutes at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin ａnd mounted with DPX.
-
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the antibody (EM1801-21) at 1/50 dilution, for 30 minutes at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin ａnd mounted with DPX.
| Positive Control | SiHa, A549, rat uterus tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000: IHC:1:50-1:100: |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
