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              技術文章

              NQO1 Antibody Catalog Number:OM286504

              點擊次數:39 發布時間:2024/9/24 14:53:57
               

              Product Profile

              Product Name NQO1 Antibody
              Antibody Type Primary Antibodies
              Product description
              NQO1 is a member of the NAD(P)H dehydrogenase(qui) family and a cylasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quis to hydroquis. This protein's enzymatic activity prevents the one electron reduction of quis that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).1) Hubackova,M., Vaclavikova,R. Pharmacogenet. Genomics 19 (7), 505-512 (2009) Canova,C., Hashibe,M. Cancer Res. 69 (7), 2956-2965 (2009) Li,Y. and Jaiswal,A.K. J. Biol. Chem. 267 (21), 15097-15104 (1992)
              More 
              Immunogen
              This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of human NQO1.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              FACSIFWB

               
              For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
              Species Reactivity

              Human

              Concentration 1 mg/ml

              Target Information

              Gene Symbol NQO1
              Alternative Names
              NAD(P)H dehydrogenase [qui] 1
              Azoreductase
              DT-diaphorase
              DTD
              Menadione reductase
              NAD(P)H:qui oxidoreductase 1
              Phylloqui reductase
              Qui reductase 1
              QR1
              NQO1
              DIA4
              NMOR1
              More 
              Molecular Weight(MW) 31 kDa

              Database Links

              Entrez Gene 1728
              Protein Accession P15559

              Application

              • Application

                Confocal immunofluorescent analysis of NQO1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

              • Application

                Western blot analysis of NQO1 antibody in NCI-H460 cell line lysates (35ug/lane)

              • Application

                Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

              Application Notes
              For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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