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聯系人：楊燕燕
技術文章
PPP1A antibody Catalog Number：OM269376
Product Profile
| Product Name | PPP1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP1A. The exact sequence is proprietary. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Dog, Pig, Rabbit, BovineMouse (99%), Xenopus laevis (97%), Dog (100%), Pig (100%), Rabbit (97%), Bovine (100%) |
| Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (2) |
| Cellular Localization | Cylasm |
Application
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Application
WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
-
Application
PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, ａnd the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
-
Application
PPP1A antibody detects PPP1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, ａnd the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
-
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
-
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A antibody at 1:100 dilution.
| Positive Control | 293T , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
原創作者：上海篤瑪生物科技有限公司
