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              技術文章

              DLK1 Antibody Catalog Number:OM277108

              點擊次數:35 發布時間:2024/9/23 15:17:19
                

              Product Profile

              Product Name DLK1 Antibody
              Antibody Type Primary Antibodies
              Product description
              DLK1 Antibody: The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.1) Smas CM and Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993; 73:725-34.
              More 
              Immunogen
              DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1. The immunogen is located within amino acids 260 - 310 of DLK1.

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              ELISAICCIFWB

               
              DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.:
              Species Reactivity

              HumanMouseRat

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol DLK1
              Alternative Names
              DLK1 Antibody: DLK
              Protein delta homolog 1
              pG2
              DLK-1
              More 
              Molecular Weight(MW) 42 kDa
              Sequence Similarities 2) Cat. No. 17-011 - Hep G2 Cell Slide
              Tissue Specificity At least four isoforms of DLK1 are known to exist.

              Database Links

              Entrez Gene 8788
              Protein Accession NP_003827

              Application

              • Application

                Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μg/mL.

              • Application

                Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μg/ml.

              • Application

                Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μg/ml.

              Positive Control 1) Cat. No. 1211 - HepG2 Cell Lysate
              Application Notes
              DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.:

              Additional Information

              Form Liquid
              Storage Instructions DLK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer DLK1 Antibody is supplied in PBS containing 0.02% sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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