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              技術文章

              TUBA8 [D7-D6] Catalog Number:OM627690

              點擊次數:26 發布時間:2024/9/23 14:57:43
                

              Product Profile

              Product Name TUBA8 [D7-D6]
              Antibody Type Primary Antibodies
              Product description
              Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
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              Immunogen
              Recombinant protein

              Key Feature

              Clonality Monoclonal
              Isotype IgG2b
              Host Species Mouse
              Tested Applications

              WBICCIHCFC

               
              WB:1:500-1:2,000
              ICC:1:100-1:500
              IHC:1:100-1:500
              FC:1:100-1:200
              More 
              Species Reactivity

              HumanRat

              Concentration 2 mg/mL.

              Target Information

              Alternative Names
              Alpha tubulin 8 antibody Alpha-tubulin 8 antibody TBA8_HUMAN antibody Tuba8 antibody TUBAL2 antibody Tubulin alpha 8 chain antibody Tubulin alpha chain like 2 antibody Tubulin alpha chain-like 2 antibody Tubulin alpha-8 chain antibody
              Molecular Weight(MW) 50 kDa
              Cellular Localization Cytoskeleton.

              Database Links

              SwissProt ID Q9NY65
              Q6AY56

              Application

              • Application

                Fig1: Western blot analysis of TUBA8 on human TUBA8 recombinant protein using anti-TUBA8 antibody at 1/1,000 dilution.

              • Application

                Fig2: Western blot analysis of TUBA8 on at heart tissue lysate using anti-TUBA8 antibody at 1/1,000 dilution.

              • Application

                Fig3: ICC staining TUBA8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

              • Application

                Fig4: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues?using anti-TUBA8 antibody. Counter stained with hematoxylin.

              • Application

                Fig5: Immunohistochemical analysis of paraffin-embedded 爈iver cancer tissues?using anti-TUBA8 antibody. Counter stained with hematoxylin.

              • Application

                Fig6: Flow cytometric analysis of NIH/3T3 cells with TUBA8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

              Positive Control Human TUBA8 recombinant protein, rat heart tissue lysate, medulla oblongata tissue, liver cancer tissue, Hela, NIH/3T3.
              Application Notes
              WB:1:500-1:2,000
              ICC:1:100-1:500
              IHC:1:100-1:500
              FC:1:100-1:200
              More 

              Additional Information

              Form Liquid
              Storage Instructions Store at +4℃after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
              Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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