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聯系人：楊燕燕
技術文章
Bmi1 [B3-G5] Catalog Number：OM628508
Product Profile
| Product Name | Bmi1 [B3-G5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within human Bmi1 full sequence. |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000 More IHC:1:200 ICC:1:200 FC:1:100-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 37kDa |
| Cellular Localization | Nucleus, Cylasm |
Database Links
| SwissProt ID | P35226 P25916 |
|---|
Application
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Application
Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.Positive control:Lane 1: 293T Lane 2: JurkatLane 3: Hela Lane 4: MCF-7Lane 5: HepG2 Lane 6: NIH/3T3Lane 7: PC12 Lane 8: Mouse kidneyLane 9: Human kidney Lane 10: K562Lane 11: Human brain
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Application
Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig4: ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
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Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
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Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
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Application
Fig8: Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
| Positive Control | Hela, HepG2, Jurkat, 293T, MCF-7, PC12, K562, NIH/3T3, A549, LOVO, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, mouse kidney tissue, human kidney tissue, human brain tissue. |
|---|---|
| Application Notes | WB:1:1,000 More IHC:1:200 ICC:1:200 FC:1:100-1:200 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
