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聯系人：楊燕燕
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技術文章
Bmi1 [JJ093-3] Catalog Number：OM637714
Product Profile
| Product Name | Bmi1 [JJ093-3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanRat |
| Concentration | 1 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Nucleus, Cylasm. |
Database Links
| SwissProt ID | P35226 307151 |
|---|
Application
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Application
Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: PC-12
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Application
Fig2: ICC staining Bmi1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig3: ICC staining Bmi1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig4: ICC staining Bmi1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
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Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
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Application
Fig7: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
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Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
| Positive Control | A549, SW480, Hela, K562, PC-12, rat smooth muscle tissue, human tonsil tissue, human lung cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
