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              技術文章

              BMI1 Catalog Number:OM640906

              點擊次數:46 發布時間:2024/9/20 15:45:53
               
              Antibody Type Primary Antibodies
              Immunogen
              Purified recombinant fragment of human BMI1 expressed in E. Coli.

              Key Feature

              Clonality Monoclonal
              Host Species Unknow
              Tested Applications

              WBIHCICCFCMICC/IF

               
              WB:1:250~1:1000
              IHC:1:20~1:100
              ICC:1:100~1:200
              ICC/IF:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 
              Species Reactivity

              Human

              Target Information

              Alternative Names
              PCGF4
              RNF51
              MGC12685
              FLVI2/BMI1
              More 
              Molecular Weight(MW) 37kDa

              Database Links

              Entrez Gene 648

              Application

              • Application

                Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

              • Application

                Figure 1: Western blot analysis using BMI1 mAb against human BMI1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)

              • Application

                Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining.

              • Application

                Figure 2: Western blot analysis using BMI1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.

              • Application

                Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 mouse mAb with DAB staining.

              • Application

                Figure 4: Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

              • Application

                Figure 5: Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).

              Application Notes
              WB:1:250~1:1000
              IHC:1:20~1:100
              ICC:1:100~1:200
              ICC/IF:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 

              Additional Information

              Form Ascitic fluid containing 0.03% sodium azide.
              Storage Instructions 4℃; -20℃ for long term storage

              原創作者:上海篤瑪生物科技有限公司

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