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聯系人：楊燕燕
技術文章
Bmi1 antibody Catalog Number：OM241672
點擊次數：92 發布時間：2024/9/20 15:43:52
Product Profile
| Product Name | Bmi1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Cat, Pig, Chicken, Chimpanzee, BovineMouse (96%), Rat (92%), Xenopus laevis (82%), Dog (98%), Cat (99%), Pig (99%), Chicken (94%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Target | Bmi1 |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Nucleus , Cylasm |
Application
-
Application
Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg THP-1 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody dilution: 1:3000
-
Application
Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody 5 % SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
| Positive Control | K562 , THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
原創作者：上海篤瑪生物科技有限公司
