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              技術文章

              NF-L antibody , C-term Catalog Number:OM265300

              點擊次數:23 發布時間:2024/9/20 15:19:01
               

              Product Profile

              Product Name NF-L antibody , C-term
              Antibody Type Primary Antibodies
              Product description
              Rabbit Monoclonal antibody [EP675Y] to NF-LNeuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton . NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) . NF-L self polymerizes and provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M . NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons .
              More 
              Immunogen
              A synthetic peptide corresponding to residues near the C-term of human NF-L was used as immunogen.

              Key Feature

              Clonality Monoclonal
              Isotype IgG
              Clone Number EP675Y
              Host Species Rabbit
              Tested Applications

              FACSIPWB

               
              For WB: Use at a dilution of 1:1,000:
              Species Reactivity

              HumanMouseRat

              Concentration 1 mg/ml

              Target Information

              Gene Synonyms
              NF68
              NEFL
              CMT2E
              NFL
              CMT1F
              NF-L
              FLJ53642
              NF L
              More 
              Target NF-L
              Molecular Weight(MW) 68 (note)

              Application

              • Application

                A. Western blot analysis on SH-SY5Y lysate using anti-NF-L RabMAb (cat. # ) dilution 1:50000

              Application Notes
              For WB: Use at a dilution of 1:1,000:

              Additional Information

              Form Liquid
              Storage Instructions Store at -20 °C. Stable for 12 months from date of receipt.
              Storage Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

              原創作者:上海篤瑪生物科技有限公司

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