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聯系人：楊燕燕
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技術文章
NF-L antibody Catalog Number：OM265292
Product Profile
| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Native NF-L purified from pig spinal cord |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| For IHC-Fr: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:1,000. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.: | |
| Species Reactivity | HumanMousePigRatMammalian speciesBovine |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Target | NF-L |
| Tissue Specificity | Recognizes the light neurofilament subunit (~68 kDa). |
Application
-
Application
Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody - Mixed neuron/glia cultures from newborn rat brain stained antibody to peripherin (green) ａnd rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons ａnd their processes contain NF-L ａnd not peripherin. Interestingly, the periperin positive cells often contain a cylasmic inclusion next to the nucleus which stains for both peripherin ａnd NF-L, ａnd so appears golden in this kind of image. The blue channel reveals the localization of DNA
-
Application
Western Blot: Neurofilament light chain Antibody - Western blot of whole rat cerebellum homogenate stained with , at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L. Human, cow ａnd bovine NF-L run a little slower, at about 70kDa.
| Positive Control | Rat cerebellum |
|---|---|
| Application Notes | For IHC-Fr: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:1,000. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.: |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot ａnd store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Whole antiserum containing no preservative |
原創作者：上海篤瑪生物科技有限公司
