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聯系人:楊燕燕
技術文章
NF-L antibody Catalog Number:OM265293
Product Profile
Product Name | NF-L antibody |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen | Recombinant full length protein (Rat) expressed in and purified from E. coli. |
Key Feature
Clonality | Monoclonal |
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Isotype | IgG |
Clone Number | 1D2 |
Host Species | Mouse |
Tested Applications | ICC/IFIHC-FIHC-PWB |
IF: Use at a dilution of 1/500. IHC-Fr: Use at a dilution of 1/5000 (formalin fixed, ABC method). IHC-P: Use at a dilution of 1/5000 (ABC method). WB: Use at a dilution of 1/10000. Detects a band of approximately 66 kDa. Optimal dilutions/concentrations should be determined by the end user.: More | |
Species Reactivity | HumanRatMammalian species |
Concentration | 1 mg/ml |
Target Information
Gene Synonyms | More |
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Target | NF-L |
Application
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Application
Mixed cultures of rat CNS cells stained with (red) and GeneTex chicken antibody to Microtubule associated protein 2 (MAP2- green, using antibody ). Thea-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.
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Application
Left three lanes show Coomassie brilliant blue stained extracts of rat brain stem, cat cerebral cortex and human cerebral cortex (R, C and H respectively). Right three lanes are corresponding blots reacted with stains a band of about 66kDa in all three preparations.
Application Notes | IF: Use at a dilution of 1/500. IHC-Fr: Use at a dilution of 1/5000 (formalin fixed, ABC method). IHC-P: Use at a dilution of 1/5000 (ABC method). WB: Use at a dilution of 1/10000. Detects a band of approximately 66 kDa. Optimal dilutions/concentrations should be determined by the end user.: More |
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Additional Information
Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Preservative: 5mM Sodium Azide; Constituents: Tissue Culture Supernatant |
原創作者:上海篤瑪生物科技有限公司