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              技術文章

              Neurofilament NF-L Antibody Catalog Number:OM286038

              點擊次數:79 發布時間:2024/9/19 14:33:07
               

              Product Profile

              Product Name Neurofilament NF-L Antibody
              Antibody Type Primary Antibodies
              Product description
              Neurofilaments are the 10nm or intermediate filament proteins specifically in neurons, and are composed predominantly of three major proteins called NF- NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.1) Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
              More 
              Immunogen
              Preparation of porcine intermediate filaments.

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Host Species Mouse
              Tested Applications

              IHCWB

               
              IHC:1:50~1:200
              WB:1:500~1:2000
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              Species Reactivity

              BovineChickenHumanMouseRat

              Concentration 1 mg/ml

              Target Information

              Gene Symbol NEFL
              Alternative Names
              NFL
              NF-L
              NF68
              CMT1F
              CMT2E
              NFL
              68 kDa neurofilament protein
              More 
              Molecular Weight(MW) 68
              Tissue Specificity Specific for the ~68k Neurofilament L protein.

              Database Links

              Entrez Gene 4747
              Protein Accession P07196

              Application

              • Application

                Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein.

              Application Notes
              IHC:1:50~1:200
              WB:1:500~1:2000
              Notes:Optimal dilutions/concentrations should be determined by the researcher.

              Additional Information

              Form Liquid
              Storage Instructions Neurofilament NF-L antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
              Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
               

              原創作者:上海篤瑪生物科技有限公司

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