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              聯系人:楊燕燕

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              技術文章

              NEFL Catalog Number:OM640884

              點擊次數:113 發布時間:2024/9/19 14:30:37
               

              Product Profile

              Product Name NEFL
              Antibody Type Primary Antibodies
              Immunogen
              Purified recombinant fragment of human NEFL expressed in E. Coli.

              Key Feature

              Clonality Monoclonal
              Host Species Unknow
              Tested Applications

              WBIHCICCFCMICC/IF

               
              WB:1:250~1:1000
              IHC:1:20~1:100
              ICC:1:100~1:200
              ICC/IF:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 
              Species Reactivity

              Human

              Target Information

              Alternative Names
              NFL
              NF-L
              NF68
              CMT1F
              CMT2E
              More 
              Molecular Weight(MW) 62kDa

              Database Links

              Entrez Gene 4747

              Application

              • Application

                Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

              • Application

                Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)

              • Application

                Figure 2: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.

              • Application

                Figure 3: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

              • Application

                Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.

              • Application

                Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.

              • Application

                Figure 4: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).

              Application Notes
              WB:1:250~1:1000
              IHC:1:20~1:100
              ICC:1:100~1:200
              ICC/IF:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 

              Additional Information

              Form Ascitic fluid containing 0.03% sodium azide
              Storage Instructions 4℃; -20℃ for long term storage
               

              原創作者:上海篤瑪生物科技有限公司

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