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              技術文章

              Fibrinogen gamma antibody Catalog Number:OM253133

              點擊次數:77 發布時間:2024/9/19 14:19:17
               

              Product Profile

              Product Name Fibrinogen gamma antibody
              Antibody Type Primary Antibodies
              Product description
              Rabbit polyclonal antibody to Fibrinogen gamma (fibrinogen gamma chain)The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]
              More 
              Immunogen
              Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              ICC/IFIHC-PWB

               
              *Optimal dilutions/concentrations should be determined by the researcher. :
              Species Reactivity

              HumanMouse

              Predicted Reactivity Rat, Pig, Chicken, BovineRat (91%), Pig (88%), Chicken (81%), Bovine (88%)
              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification Affinity purified

              Target Information

              Gene Synonyms
              FGG antibody
              "fibrinogen
              gamma polypeptide antibody"
              fibrinogen gamma chain antibody
              More 
              Target Fibrinogen gamma
              Molecular Weight(MW) 52 kDa (note) (3)
              Cellular Localization Secreted

              Application

              • Application

                Fibrinogen gamma antibody detects Fibrinogen gamma protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted by 1:1000.

              • Application

                Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrinogen gamma antibody at 1:200 dilution.

              • Application

                Fibrinogen gamma antibody detects Fibrinogen gamma protein by western blot analysis. Human plasma and HepG2 conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:2000.

              • Application

                Fibrinogen gamma antibody detects Fibrinogen gamma protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Fibrinogen gamma antibody dilution: 1:250.

              • Application

                Fibrinogen gamma antibody detects Fibrinogen gamma protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:1000.

              Positive Control 293T , A431 , HeLa , HepG2 , human plasma , HepG2 sup. , Mouse plasma
              Application Notes
              *Optimal dilutions/concentrations should be determined by the researcher. :

              Additional Information

              Form Liquid
              Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

              原創作者:上海篤瑪生物科技有限公司

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