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              技術文章

              FIBG Antibody Catalog Number:OM278858

              點擊次數:43 發布時間:2024/9/19 11:55:50
               

              Product Profile

              Product Name FIBG Antibody
              Antibody Type Primary Antibodies
              Product description
              FIBG is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.1) Nowak-Gottl,U., Blood 114 (9), 1947-1953 (2009)
              More 
              Immunogen
              This FIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-449 amino acids from the C-terminal region of human FIBG.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              IFIHC-PWB

               
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
              Species Reactivity

              Human

              Concentration 1 mg/ml

              Target Information

              Gene Symbol FGG
              Alternative Names
              Fibrinogen gamma chain
              FGG
              Molecular Weight(MW) 52 kDa

              Database Links

              Entrez Gene 2266
              Protein Accession P02679

              Application

              • Application

                Confocal immunofluorescent analysis of FIBG Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

              • Application

                FIBG Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

              • Application

                Western blot analysis of FIBG Antibody in NCI-H460 cell line lysates (35ug/lane)

              • Application

                Western Blot at 1:4000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.

              Application Notes
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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