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              技術文章

              ALPL Antibody (Center) Catalog Number:OM226338

              點擊次數:37 發布時間:2024/9/18 14:34:16
               

              Product Profile

              Product Name ALPL Antibody (Center)
              Antibody Type Primary Antibodies

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              WBIFFC

               
              WB~~1:100~500 IF~~1:10~50 FC~~1:10~50:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification  

              Target Information

              Alternative Names
              ALPL
              Alkaline phosphatase
              tissue-nonspecific isozyme
              Alkaline phosphatase liver/bone/kidney isozyme
              More 
              Molecular Weight(MW) 57305 Da
              Function
              This isozyme may play a role in skeletal mineralization.
              Tissue Specificity This ALPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~254 amino acids from the Center region of human ALPL.
              Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor.

              Database Links

              Entrez Gene 249

              Application

              • Application

                Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene.

              • Application

                ALPL Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ALPL antibody detected the ALPL protein (arrow).

              • Application

                Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat#AP1474c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

              • Application

                ALPL Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

              Application Notes
              WB~~1:100~500 IF~~1:10~50 FC~~1:10~50:

              Additional Information

              Form Liquid
              Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

              原創作者:上海篤瑪生物科技有限公司

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