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              技術文章

              RPA1 Antibody Catalog Number:OM290268

              點擊次數:27 發布時間:2024/9/18 13:16:28
               

              Product Profile

              Product Name RPA1 Antibody
              Antibody Type Primary Antibodies
              Product description
              RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.1) Choi, J.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13660-13665(2010)
              More 
              Immunogen
              This RPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-559 amino acids from the C-terminal region of human RPA1.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              WB

               
              For WB starting dilution is: 1:1000:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol RPA1
              Alternative Names
              Replication protein A 70 kDa DNA-binding subunit
              RP-A p70
              Replication factor A protein 1
              RF-A protein 1
              Single-stranded DNA-binding protein
              Replication protein A 70 kDa DNA-binding subunit
              N-terminally processed
              RPA1
              REPA1
              RPA70
              More 
              Molecular Weight(MW) 68 kDa

              Database Links

              Entrez Gene 6117
              Protein Accession P27694

              Application

              • Application

                Western blot analysis in K562 cell line lysates (35ug/lane).

              Application Notes
              For WB starting dilution is: 1:1000:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
               

              原創作者:上海篤瑪生物科技有限公司

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