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              技術文章

              eIF4B (phospho Ser422) Antibody Catalog Number:OM277787

              點擊次數:23 發布時間:2024/9/13 13:46:28
               

              Product Profile

              Product Name eIF4B (phospho Ser422) Antibody
              Antibody Type Primary Antibodies
              Product description
              Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.1) Gingras, A. C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. and Hershey, J. W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R. F. and Hershey, J. W. (1989) J. Cell Biol. 109, 1467-1481.
              More 
              Immunogen
              eIF4B (phospho-Ser422) antibody was raised against a peptide sequence around phosphorylation site of Serine 422 (T-G-S (p) -E-S) derived from Human eIF4B.

              Key Feature

              Clonality Polyclonal
              Host Species Rabbit
              Tested Applications

              IHCWB

               
              Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
              Species Reactivity

              HumanMouse

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol EIF4B
              Alternative Names
              EIF-4B
              PRO1843
              ESR
              NR3A1
              More 
              Molecular Weight(MW) 80 kDa
              Tissue Specificity This antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.

              Database Links

              Entrez Gene 1975
              Protein Accession NP_001408.2

              Application

              • Application

                Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422).

              • Application

                Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using eIF4B (phospho-Ser422).

              Application Notes
              Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

              Additional Information

              Form Liquid
              Storage Instructions Store antibody at -20˚C for up to one year.
              Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

              原創作者:上海篤瑪生物科技有限公司

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