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技術文章
EIF4B [D7-F5] Catalog Number：OM627752
點擊次數：22 發布時間：2024/9/13 13:45:35
Product Profile
| Product Name | EIF4B [D7-F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 More ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytosol. |
Database Links
| SwissProt ID | P23588 Q8BGD9 |
|---|
Application
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Application
Fig1: Western blot analysis of EIF4B on human EIF4B recombinant protein using anti-EIF4B antibody at 1/1,000 dilution.
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Application
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Application
Fig3: ICC staining EIF4B (green) ａnd actin filaments (red) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-EIF4B antibody. Counter stained with hematoxylin.
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Application
Fig5: Flow cytometric analysis of Hela cells with EIF4B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
| Positive Control | Human EIF4B recombinant protein, A431, A549, HepG2, HEK293, HeLa, Jurkat, K562, NIH-3T3, MCF-7, colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 More ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
