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              技術文章

              eIF4B (phospho Ser422) antibody Catalog Number:OM251211

              點擊次數:20 發布時間:2024/9/13 13:42:49
               

              Product Profile

              Product Name eIF4B (phospho Ser422) antibody
              Antibody Type Primary Antibodies
              Product description
              Rabbit Polyclonal antibody to eIF4B (Phospho Ser422)Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
              More 
              Immunogen
              Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B.

              Key Feature

              Clonality Polyclonal
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
              Species Reactivity

              HumanMouse

              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification Affinity purified

              Target Information

              Gene Synonyms
              EIF-4B
              PRO1843
              EIF4B
              eukaryotic translation initiation factor 4B
              More 
              Target eIF4B (Phospho Ser422)
              Molecular Weight(MW) 80 (note)

              Application

              • Application

                Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B (phospho-Ser422) Antibody

              • Application

                Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422) Antibody (left) or the same antibody preincubated with blocking peptide (right).

              • Application

                Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B (phospho-Ser422) Antibody .

              Application Notes
              Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

              Additional Information

              Form Liquid
              Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

              原創作者:上海篤瑪生物科技有限公司

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