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              技術文章

              eIF4B antibody Catalog Number:OM251212

              點擊次數:21 發布時間:2024/9/13 13:42:05
               

              Product Profile

              Product Name eIF4B antibody
              Antibody Type Primary Antibodies
              Product description
              Rabbit Polyclonal antibody to eIF4BRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
              More 
              Immunogen
              Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.

              Key Feature

              Clonality Polyclonal
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
              Species Reactivity

              Human

              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification Affinity purified

              Target Information

              Gene Synonyms
              EIF-4B
              PRO1843
              EIF4B
              eukaryotic translation initiation factor 4B
              More 
              Target eIF4B
              Molecular Weight(MW) 80 (note)

              Application

              • Application

                Western blot analysis of extracts from 3T3 and HL60 cells using eIF4B(Ab-422) Antibody

              • Application

                Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B Antibody (left) or the same antibody preincubated with blocking peptide (right).

              Application Notes
              Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

              Additional Information

              Form Liquid
              Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

              原創作者:上海篤瑪生物科技有限公司

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