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              技術文章

              EIF4B Antibody Catalog Number:OM277788

              點擊次數:20 發布時間:2024/9/13 13:38:54
               

              Product Profile

              Product Name EIF4B Antibody
              Antibody Type Primary Antibodies
              Product description
              IF4B contains 1 RRM (RNA recognition motif) domain and is required for the binding of mRNA to ribosomes. Functions of EIF4B are in close association with EEF4-F and EIF4-A. It binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP and promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.1) Suzuki, Y., (2004) Genome Res. 14 (9), 1711-1718.
              More 
              Immunogen
              Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4B.

              Key Feature

              Clonality Polyclonal
              Host Species Rabbit
              Tested Applications

              ELISAIHCWB

               
              EIF4B antibody can be used for detection of EIF4B by ELISA at 1:62500. EIF4B antibody can be used for detection of EIF4B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification Protein A

              Target Information

              Gene Symbol EIF4B
              Alternative Names
              EIF4B
              EIF-4B
              PRO1843
              Molecular Weight(MW) 67 kDa

              Database Links

              Entrez Gene 1975
              Protein Accession NP_001408

              Application

              • Application

                Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.

              • Application

                Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.

              Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
              Application Notes
              EIF4B antibody can be used for detection of EIF4B by ELISA at 1:62500. EIF4B antibody can be used for detection of EIF4B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

              Additional Information

              Form Liquid
              Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF4B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
              Storage Buffer IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

              原創作者:上海篤瑪生物科技有限公司

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