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              技術文章

              EIF4B Antibody Catalog Number:OM277792

              點擊次數:23 發布時間:2024/9/13 13:37:17
               

              Product Profile

              Product Name EIF4B Antibody
              Antibody Type Primary Antibodies
              Product description
              EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
              More 
              Immunogen
              This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-382 amino acids from the Central region of human EIF4B.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
              Species Reactivity

              Human

              Concentration 1 mg/ml

              Target Information

              Gene Symbol EIF4B
              Alternative Names
              Eukaryotic translation initiation factor 4B
              eIF-4B
              EIF4B
              Molecular Weight(MW) 69 kDa
              Sequence Similarities Predicted species reactivity based on immunogen sequence: Mouse

              Database Links

              Entrez Gene 1975
              Protein Accession P23588

              Application

              • Application

                Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

              • Application

                Western blot analysis of anti-EIF4B Antibody Pab in HepG2 cell line lysates (35ug/lane).

              • Application

                Western blot analysis of anti-EIF4B Antibody Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane).

              • Application

                Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

              Application Notes
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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