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              技術文章

              MAPK8 Antibody Catalog Number:OM284173

              點擊次數:26 發布時間:2024/9/12 15:14:09
               

              Product Profile

              Product Name MAPK8 Antibody
              Antibody Type Primary Antibodies
              Product description
              JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.1) Deng, Y., et al., Cell 115(1):61-70 (2003).
              More 
              Immunogen
              This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 amino acids from the C-terminal region of human MAPK8.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              IFIHC-PWB

               
              For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
              Species Reactivity

              Human

              Concentration 1 mg/ml

              Target Information

              Gene Symbol MAPK8
              Alternative Names
              Mitogen-activated protein kinase 8
              MAP kinase 8
              MAPK 8
              JNK-46
              Stress-activated protein kinase 1c
              SAPK1c
              Stress-activated protein kinase JNK1
              c-Jun N-terminal kinase 1
              MAPK8
              JNK1
              PRKM8
              SAPK1
              SAPK1C
              More 
              Molecular Weight(MW) 48 kDa
              Sequence Similarities Predicted species reactivity based on immunogen sequence: Rat

              Database Links

              Entrez Gene 5599
              Protein Accession P45983

              Application

              • Application

                MAPK8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

              • Application

                Western blot analysis of anti-JNK1 Pab in HL60 (UV-treated) cell lysate

              • Application

                Confocal immunofluorescent analysis of MAPK8 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

              Application Notes
              For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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