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技術文章
MELK [B7-G2] Catalog Number：OM627794
點擊次數：25 發布時間：2024/9/12 14:56:14
Product Profile
| Product Name | MELK [B7-G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFICCIHCFC |
| ICC:1:200-1:1000 IHC:1:200-1:1,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 75kDa |
| Cellular Localization | Cell membrane. |
Database Links
| SwissProt ID | Q14680 |
|---|
Application
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Application
Fig1: ICC staining MELK (green) ａnd actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.
-
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.
-
Application
Fig4: Flow cytometric analysis of MCF-7 cells with MELK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
| Positive Control | HepG2, human liver cancer tissue, human rectum cancer tissue, MCF-7. |
|---|---|
| Application Notes | ICC:1:200-1:1000 IHC:1:200-1:1,000 FC:1:100-1:200 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
