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              技術文章

              Rabbit anti-MELK polyclonal antibody - middle region Catalog Number:OM112441

              點擊次數:26 發布時間:2024/9/12 14:40:42
               

              Product Profile

              Product Name Rabbit anti-MELK polyclonal antibody - middle region
              Antibody Type Primary Antibodies
              Immunogen
              The immunogen for anti-MELK antibody: synthetic peptide directed towards the middle region of human MELK

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              WB

               
              WB:1:500~1:2000
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              Species Reactivity

              BovineDogGuinea PigHorseHumanPigRabbit

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol MELK
              Gene Synonyms
              HPK38
              KIAA0175
              Gene Full Name Maternal embryonic leucine zipper kinase
              Gene Summary
              The protein MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. Also it may be involved in the inhibition of spliceosome assembly during mitosis.
              Alternative Names
              HPK38
              KIAA0175
              Molecular Weight(MW) 75kDa
              Sequence 651 amino acids

              Database Links

              Entrez Gene 9833
              SwissProt ID Q14680
              Protein Accession NP_055606

              Application

              • Western blot

                Host: Rabbit
                Target Name: MELK
                Sample Tissue: OVCAR-3
                Antibody Dilution: 1.0ug/ml

              • Western blot

                Host: Rabbit
                Target Name: MELK
                Sample Tissue: HCT15
                Antibody Dilution: 1.0ug/ml

              • Western blot

                0.2-1 ug/ml
                ELISA Titer: 1:62500
                Positive Control: HT1080 cell lysate

              Application Notes
              WB:1:500~1:2000
              Notes:Optimal dilutions/concentrations should be determined by the researcher.

              Additional Information

              Form Liquid
              Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
              Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

              原創作者:上海篤瑪生物科技有限公司

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