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              技術文章

              PPM1A antibody Catalog Number:OM269348

              點擊次數:37 發布時間:2024/9/11 14:00:44
               

              Product Profile

              Product Name PPM1A antibody
              Antibody Type Primary Antibodies
              Product description
              Mouse Monoclonal Antibody [7F12] to PPM1AThe protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
              More 
              Immunogen
              The immunogen corresponding to a region within amino acids 202 and 382 of human PPM1A expressed in E. Coli.

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Clone Number 7F12
              Host Species Mouse
              Tested Applications

              ELISAFACSWB

               
              ELISA: 1/10000.:
              Species Reactivity

              HumanMonkey

              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification  

              Target Information

              Gene Synonyms
              PP2CA
              PP2CALPHA
              PP2C-ALPHA
              Target PPM1A
              Molecular Weight(MW) 42.4 kDa (note)

              Application

              • Application

                ELISA analysis of control antigen and antigen using PPM1A antibody [7F12].

              • Application

                FACS analysis of HeLa cells using PPM1A antibody [7F12] (green) or a negative control (red).

              • Application

                WB analysis of lysates from (1) untransfected and (2) PPM1A (a.a. 202-382)-hIgGFc transfected HEK293 cells using PPM1A antibody [7F12].

              • Application

                WB analysis of (1) Jurkat, (2) Jurkat, (3) A431, (4) HeLa, (5) HEK293, (6) Raji, (7) MCF-7 and (8) COS7 lysates using PPM1A antibody [7F12].

              • Application

                WB analysis of human PPM1A recombinant protein using PPM1A antibody [7F12].

              Application Notes
              ELISA: 1/10000.:

              Additional Information

              Form Liquid
              Storage Instructions 4°C; -20°C for long term storage
              Storage Buffer Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.

              原創作者:上海篤瑪生物科技有限公司

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