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              技術文章

              PPM1A antibody Catalog Number:OM269350

              點擊次數:32 發布時間:2024/9/11 13:59:48
               

              Product Profile

              Product Name PPM1A antibody
              Antibody Type Primary Antibodies
              Product description
              Mouse monoclonal antibody [p6c7] to PPM1AThe protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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              Immunogen
              Recombinant human PP2C alpha (1-382aa) purified from E. coli

              Key Feature

              Clonality Monoclonal
              Isotype IgG2
              Clone Number p6c7
              Host Species Mouse
              Tested Applications

              ELISAIPWB

               
              The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250.:
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              Species Reactivity

              HumanMouse

              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification Affinity purified

              Target Information

              Gene Synonyms
              PP2CA
              PPM1A
              protein phosphatase
              Mg2+/Mn2+ dependent
              1A
              PP2C-ALPHA
              More 
              Target PPM1A
              Light Chain Type kappa

              Application

              • Application

                WB analysis of mouse liver cytosol extract using PP2C alpha antibody at a dilution of 1:250.

              Application Notes
              The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250.:
              More 

              Additional Information

              Form Liquid
              Storage Instructions Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
              Storage Buffer Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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