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              技術文章

              ARHGDIA antibody Catalog Number:OM240098

              點擊次數:23 發布時間:2024/9/10 11:20:27
               

              Product Profile

              Product Name ARHGDIA antibody
              Antibody Type Primary Antibodies
              Product description
              Mouse monoclonal antibody [1F2] to ARHGDIARegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
              Immunogen
              Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Clone Number 1F2
              Host Species Mouse
              Tested Applications

              FACSWB

               
              IHC 1:50 :
              Species Reactivity

              HumanDogMonkeyRat

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Synonyms
              MGC117248
              GDIA1
              RHOGDI
              ARHGDIA
              More 
              Target ARHGDIA
              Tissue Specificity is specific for human ARHGDIA (NP_004300).

              Application

              • Application

                HEK293T cells transfected with either RC200902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGDIA antibody, and then analyzed by flow cytometry.

              • Application

                HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA.

              • Application

                Flow cytometric Analysis of Jurkat cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

              • Application

                Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

              • Application

                Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.

              Application Notes
              IHC 1:50 :

              Additional Information

              Form Liquid
              Storage Instructions For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.
              Storage Buffer PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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