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              技術文章

              ARHGDIA Antibody Catalog Number:OM271535

              點擊次數:21 發布時間:2024/9/10 11:19:34
               

              Product Profile

              Product Name ARHGDIA Antibody
              Antibody Type Primary Antibodies
              Product description
              ARHGDIA belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA1) Qiao,J., etc, Am. J. Physiol., Cell Physiol. 295 (5), C1161-C1168 (2008)
              More 
              Immunogen
              This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 amino acids from the N-terminal region of human ARHGDIA.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000:
              Species Reactivity

              HumanMouse

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol ARHGDIA
              Alternative Names
              Rho GDP-dissociation inhibitor 1
              Rho GDI 1
              Rho-GDI alpha
              ARHGDIA
              GDIA1
              More 
              Molecular Weight(MW) 23 kDa
              Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine

              Database Links

              Entrez Gene 396
              Protein Accession P52565

              Application

              • Application

                Western blot analysis in A2058,Hela cell line and mouse lung lysates (35ug/lane).

              • Application

                Immunohistochemical analysis of paraffin-embedded H.liver section using ARHGDIA Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

              Application Notes
              For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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