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              技術文章

              CSNK2A2 [A10-E1] Catalog Number:OM627786

              點擊次數:23 發布時間:2024/9/9 14:01:02
               

              Product Profile

              Product Name CSNK2A2 [A10-E1]
              Antibody Type Primary Antibodies
              Product description
              CSNK2A2 also named CKII. Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iα, Iγ, Iδ and Ie, have been implicated in the control of cylasmic and nuclear processes, including DNA replication and repair. Casein kinase II is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The a catalytic subunit is stimulated by the β regulatory subunit, which undergoes auhosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.
              More 
              Immunogen
              Recombinant protein

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Host Species Mouse
              Tested Applications

              WBFC

               
              WB:1:500-1:1,000
              FC:1:100-1:200
              Species Reactivity

              HumanMouseRat

              Concentration 2 mg/mL.

              Target Information

              Alternative Names
              Casein kinase 2 alpha prime polypeptide antibody Casein kinase II alpha' chain antibody Casein kinase II subunit alpha' antibody CK II alpha' antibody CK II antibody CK2A2 antibody CKII antibody CSK22_HUMAN antibody CSNK2A1 antibody CSNK2A2 antibody FLJ43934 antibody
              Molecular Weight(MW) 41 kDa
              Cellular Localization Cytosol. Nucleus.

              Database Links

              SwissProt ID P19784
              O54833
              B4F7A9

              Application

              • Application

                Fig1: Western blot analysis of CSNK2A2 on human CSNK2A2 recombinant protein using anti-CSNK2A2 antibody at 1/1,000 dilution.

              • Application

                Fig2: Western blot analysis of CSNK2A2 on different cell lysates using anti-CSNK2A2 antibody at 1/1,000 dilution. Positive control: Lane1: HeLa Lane2: MCF-7 Lane3: HepG2 Lane4: Jurkat Lane5: NIH-3T3 Lane6: PC-12

              • Application

                Fig3: Flow cytometric analysis of HepG2 cells with CSNK2A2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

              Positive Control Human CSNK2A2 recombinant protein, HeLa, MCF-7, HepG2, Jurkat, NIH-3T3, PC-12.
              Application Notes
              WB:1:500-1:1,000
              FC:1:100-1:200

              Additional Information

              Form Liquid
              Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
              Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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