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聯系人：楊燕燕
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技術文章
Annexin A1 antibody Catalog Number：OM239514
Product Profile
| Product Name | Annexin A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, Rabbit, Chimpanzee, BovineDog (91%), Pig (88%), Rabbit (91%), Chimpanzee (100%), Bovine (90%) |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Nucleus , Cylasm , Cell projection , cilium , Basolateral cell membrane |
Application
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Application
Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody . Western blot analysis was performed using Annexin A1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
-
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin I antibody at 1:200 dilution.
-
Application
Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I antibody at 1:500 dilution.
-
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
-
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
-
Application
Wild-type (WT) ａnd Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, ａnd the membrane was blotted with Annexin A1 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
-
Application
Annexin A1 antibody detects Annexin A1 protein at cytosol ａnd nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 antibody dilution: 1:500.
| Positive Control | A431 , H1299 , HeLa , Molt-4 , NIH-3T3 , JC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
原創作者：上海篤瑪生物科技有限公司
