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技術文章
MARK3 [H12-G2] Catalog Number：OM627647
點擊次數：32 發布時間：2024/9/5 14:26:00
Product Profile
| Product Name | MARK3 [H12-G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 More IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 84 kDa |
| Cellular Localization | Cell membrane. Peripheral membrane protein. |
Database Links
| SwissProt ID | P27448 |
|---|
Application
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Application
Fig1: Western blot analysis of MARK3 on human MARK3 recombinant protein using anti-MARK3 antibody at 1/1,000 dilution.
-
Application
Fig2: Western blot analysis of MARK3 on different cell lysate using anti-MARK3 antibody at 1/1,000 dilution. Positive control：Line1:HeLaLine2:SK-N-SHLine3:K562Line4:HCT116Line5:HEK293Line6:3T3L1Line7:NIH3T3Line8:JurkatLine9:A431
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Application
Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-MARK3 antibody. Counter stained with hematoxylin.
-
Application
Fig4: Flow cytometric analysis of SK-N-SH cells with MARK3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
| Positive Control | Human MARK3 recombinant protein, HeLa, SK-N-SH, K562, HCT116, HEK293, 3T3L1, NIH3T3, Jurkat, ａnd A431 cell lysate, human bladder cancer tissue, SK-N-SH. |
|---|---|
| Application Notes | WB:1:250~1:1000 More IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
