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聯系人：楊燕燕
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技術文章
CD59 Catalog Number：OM640761
Product Profile
| Product Name | CD59 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human CD59 (AA: 31-111) expressed in E. Coli. |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 14.2kDa |
Database Links
| Entrez Gene | 966 |
|---|
Application
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Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Application
Figure 1: Western blot analysis using CD59 mAb against human CD59 recombinant protein. (Expected MW is 34.7 kDa)
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Application
Figure 2: Western blot analysis using CD59 mAb against HEK293 (1) ａnd CD59 (AA: 31-111)-hIgGFc transfected HEK293 (2) cell lysate.
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Application
Figure 3: Immunofluorescence analysis of MCF-7 cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Application
Figure 4: Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Application
Figure 5: Flow cytometric analysis of HeLa cells using CD59 mouse mAb (green) ａnd negative control (red).
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Application
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD59 mouse mAb with DAB staining.
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Application
Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD59 mouse mAb with DAB staining.
| Application Notes | WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
Additional Information
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
原創作者：上海篤瑪生物科技有限公司
