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              技術文章

              CD59 antibody Catalog Number:OM245695

              點擊次數:33 發布時間:2024/9/4 15:01:06
               

              Product Profile

              Product Name CD59 antibody
              Antibody Type Primary Antibodies
              Product description
              Mouse Monoclonal Antibody [8D2B8] to CD59This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
              More 
              Immunogen
              The immunogen corresponding to a region within amino acids 31 and 111 of human CD59 expressed in E. Coli.

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Clone Number 8D2B8
              Host Species Mouse
              Tested Applications

              ELISAFACSICC/IFIHCIHC-PWB

               
              ELISA: 1/10000.:
              Species Reactivity

              Human

              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification  

              Target Information

              Gene Synonyms
              EJ16
              16.3A5
              MIC11
              HRF20
              MIN1
              MSK21
              MAC-IP
              HRF-20
              MIRL
              G344
              MACIF
              EJ30
              P18-20
              MIN3
              MIN2
              EL3
              More 
              Target CD59
              Molecular Weight(MW) 14.2 kDa (note)

              Application

              • Application

                ELISA analysis of control antigen and antigen using CD59 antibody [8D2B8].

              • Application

                WB analysis of human CD59 recombinant protein using CD59 antibody [8D2B8].

              • Application

                WB analysis of lysates from (1) untransfected and (2) CD59 (a.a. 31-111)-hIgGFc transfected HEK293 cells using CD59 antibody [8D2B8].

              • Application

                IHC-P analysis of bladder cancer tissues using CD59 antibody [8D2B8].

              • Application

                IHC-P analysis of esophageal cancer tissues using CD59 antibody [8D2B8].

              • Application

                ICC/IF analysis of MCF-7 cells using CD59 antibody [8D2B8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).

              • Application

                FACS analysis of HeLa cells using CD59 antibody [8D2B8] (green) or a negative control (red).

              • Application

                ICC/IF analysis of HeLa cells using CD59 antibody [8D2B8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).

              Application Notes
              ELISA: 1/10000.:

              Additional Information

              Form Liquid
              Storage Instructions 4°C; -20°C for long term storage
              Storage Buffer Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.

              原創作者:上海篤瑪生物科技有限公司

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