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技術文章
CD59 [JM10-71] Catalog Number：OM637851
Product Profile
| Product Name | CD59 [JM10-71] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFCIP |
| WB:1:1,000-1:5,000 More ICC:1:100-1:500 FC:1:50-1:100 IP:1:10-1:50 | |
| Species Reactivity | Human |
| Concentration | 1 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 14/20 kDa |
| Cellular Localization | Cell membrane. Secreted. |
Database Links
| SwissProt ID | P13987 |
|---|
Application
-
Application
Fig1: Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution. Positive control: Lane 1: Human placenta Lane 2: HUVEC Lane 3: K562
-
Application
Fig2: ICC staining CD59 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Application
Fig3: ICC staining CD59 in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Application
Fig4: ICC staining CD59 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Application
Fig5: Flow cytometric analysis of NIH-3T3 cells with CD59 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | NIH-3T3, HUVEC, JAR, K562, Human placenta. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 More ICC:1:100-1:500 FC:1:50-1:100 IP:1:10-1:50 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
