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              技術文章

              DKK3 [C6-A5] Catalog Number:OM627641

              點擊次數:32 發布時間:2024/9/4 13:49:38
               

              Product Profile

              Product Name DKK3 [C6-A5]
              Antibody Type Primary Antibodies
              Product description
              Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
              More 
              Immunogen
              Recombinant protein

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Host Species Mouse
              Tested Applications

              WBIHC

               
              WB:1:250~1:1000
              IHC:1:20~1:100
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              Species Reactivity

              Human

              Concentration 2 mg/mL.

              Target Information

              Alternative Names
              Dickkopf 3 antibody Dickkopf 3 homolog (Xenopus laevis) antibody Dickkopf 3 homolog antibody Dickkopf homolog 3 antibody Dickkopf related protein 3 antibody Dickkopf related protein 3 Precursor antibody Dickkopf WNT signaling pathway inhibitor 3 antibody Dickkopf
              Xenopus
              homolog of
              3 antibody Dickkopf-3 antibody Dickkopf-related protein 3 antibody Dickkopf3 antibody DKK 3 antibody Dkk-3 antibody DKK3 antibody DKK3_HUMAN antibody hDkk 3 antibody hDkk-3 antibody hDkk3 antibody Regulated in glioma antibody REIC antibody RIG antibody RIG-like 5-6 antibody RIG-like 7-1 antibody
              More 
              Molecular Weight(MW) 38 kDa
              Cellular Localization Secreted.

              Database Links

              SwissProt ID Q9UBP4

              Application

              • Application

                Fig1: Western blot analysis of DKK3 on human DKK3 recombinant protein using anti-DKK3 antibody at 1/1,000 dilution.

              • Application

                Fig2: Western blot analysis of DKK3 on HEK293 (1) and DKK3-hIgGFc transfected HEK293 (2) cell lysate using anti-DKK3 antibody at 1/1,000 dilution.

              • Application

                Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-DKK3 antibody. Counter stained with hematoxylin.

              Positive Control Human DKK3 recombinant protein, DKK3-hIgGFc transfected HEK293 cell lysate, human brain tissue.
              Application Notes
              WB:1:250~1:1000
              IHC:1:20~1:100
              Notes:Optimal dilutions/concentrations should be determined by the researcher.

              Additional Information

              Form Liquid
              Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
              Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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