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              技術文章

              DKK3 Antibody Catalog Number:OM277084

              點擊次數:28 發布時間:2024/9/4 11:58:33
               

              roduct Profile

              Product Name DKK3 Antibody
              Antibody Type Primary Antibodies
              Product description
              DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.1) Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
              More 
              Immunogen
              This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol DKK3
              Alternative Names
              Dickkopf-related protein 3
              Dickkopf-3
              Dkk-3
              hDkk-3
              DKK3
              REIC
              More 
              Molecular Weight(MW) 38 kDa

              Database Links

              Entrez Gene 27122
              Protein Accession Q9UBP4

              Application

              • Application

                Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with DKK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

              • Application

                Formalin-fixed and paraffin-embedded human brain tissue reacted with DKK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

              • Application

                Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30) at 1:1000 at each lane.

              Application Notes
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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