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              技術文章

              PDE1B Antibody Catalog Number:OM287663

              點擊次數:33 發布時間:2024/9/3 14:15:15
               

              Product Profile

              Product Name PDE1B Antibody
              Antibody Type Primary Antibodies
              Product description
              Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
              More 
              Immunogen
              This PDE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-500 amino acids from the C-terminal region of human PDE1B.

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              IHC-PWB

               
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
              Species Reactivity

              HumanMouse

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol PDE1B
              Alternative Names
              Calcium/calmodulin-dependent 3'
              5'-cyclic nucleotide phosphodiesterase 1B
              Cam-PDE 1B
              63 kDa Cam-PDE
              PDE1B
              PDE1B1
              PDES1B
              More 
              Molecular Weight(MW) 61 kDa

              Database Links

              Entrez Gene 5153
              Protein Accession Q01064

              Application

              • Application

                PDE1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

              • Application

                Western Blot at 1:1000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.

              • Application

                Western blot analysis in mouse brain tissue lysates (35ug/lane).

              Application Notes
              For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

              Additional Information

              Form Liquid
              Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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